Review



miscript mimic-mir negative control  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Qiagen miscript mimic-mir negative control
    Miscript Mimic Mir Negative Control, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+mimic-mir+negative+control/pmc07666224-233-43-48?v=Qiagen
    Average 90 stars, based on 1 article reviews
    miscript mimic-mir negative control - by Bioz Stars, 2026-08
    90/100 stars

    Images



    Similar Products

    miscript mimic-mir negative control  (Qiagen)
    90
    Qiagen miscript mimic-mir negative control
    Miscript Mimic Mir Negative Control, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+mimic-mir+negative+control/pmc07666224-233-43-48?v=Qiagen
    Average 90 stars, based on 1 article reviews
    miscript mimic-mir negative control - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    miscript mirna mimics: syn-hsa-mir-26a-5p, syn-hsa-mir-101–3p, syn-hsa-mir-93, and allstars negative control sirna  (Qiagen)
    90
    Qiagen miscript mirna mimics: syn-hsa-mir-26a-5p, syn-hsa-mir-101–3p, syn-hsa-mir-93, and allstars negative control sirna
    Up-regulated miRNAs targeting Polycomb group and associated proteins 16 of 32 WT1-induced miRNAs have putative seed sequences in the 3′ UTR of EZH2 and various Polycomb-associated proteins.
    Miscript Mirna Mimics: Syn Hsa Mir 26a 5p, Syn Hsa Mir 101–3p, Syn Hsa Mir 93, And Allstars Negative Control Sirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+mimic-mir+negative+control/pmc04759161-202-39-58?v=Qiagen
    Average 90 stars, based on 1 article reviews
    miscript mirna mimics: syn-hsa-mir-26a-5p, syn-hsa-mir-101–3p, syn-hsa-mir-93, and allstars negative control sirna - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Up-regulated miRNAs targeting Polycomb group and associated proteins 16 of 32 WT1-induced miRNAs have putative seed sequences in the 3′ UTR of EZH2 and various Polycomb-associated proteins.

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: Up-regulated miRNAs targeting Polycomb group and associated proteins 16 of 32 WT1-induced miRNAs have putative seed sequences in the 3′ UTR of EZH2 and various Polycomb-associated proteins.

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques:

    Validation of selected miRNAs. A, miR-26a levels were quantified (n = 3) by qRT-PCR with RNU6B as the reference miRNA transcript in various cell lines. B, miR-101 levels were quantified (n = 3), using RNU6B for normalization. One-way analysis of variance with Dunnett's multiple comparison tests versus parental amMSC line: p < 0.0001 (****), p < 0.001 (***), not significant (ns).

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: Validation of selected miRNAs. A, miR-26a levels were quantified (n = 3) by qRT-PCR with RNU6B as the reference miRNA transcript in various cell lines. B, miR-101 levels were quantified (n = 3), using RNU6B for normalization. One-way analysis of variance with Dunnett's multiple comparison tests versus parental amMSC line: p < 0.0001 (****), p < 0.001 (***), not significant (ns).

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques: Biomarker Discovery, Quantitative RT-PCR, Comparison

    Effect of different miRNA concentrations on exogenous EZH2. A, absence of endogenous EZH2 transcript in control human fibroblasts versus fibroblasts transfected with CMV-EZH2 expression plasmid. B, dose-response curve showing the effect of various concentrations of miR-26a on the expression of exogenous EZH2 mRNA. The minimal dose with the maximum effect is 25 nm (n = 4). C, dose-response curve showing the effect of various concentrations of miR-101 on the expression of exogenous EZH2. The minimal dose with the maximum effect is 20 nm (n = 4).

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: Effect of different miRNA concentrations on exogenous EZH2. A, absence of endogenous EZH2 transcript in control human fibroblasts versus fibroblasts transfected with CMV-EZH2 expression plasmid. B, dose-response curve showing the effect of various concentrations of miR-26a on the expression of exogenous EZH2 mRNA. The minimal dose with the maximum effect is 25 nm (n = 4). C, dose-response curve showing the effect of various concentrations of miR-101 on the expression of exogenous EZH2. The minimal dose with the maximum effect is 20 nm (n = 4).

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques: Control, Transfection, Expressing, Plasmid Preparation

    WT1 cooperates with miRNAs to inhibit EZH2 expression. A, levels of EZH2 mRNA were quantified by qRT-PCR (n = 4) and normalized to β2-microglobulin (B2M) in human fibroblasts transiently transfected with EZH2, WT1, miR-26a, miR-101, or their control plasmids. B, levels of EZH2 protein were quantified by densitometric analysis of Western immunoblots normalized for β-actin (n = 4) in human fibroblasts transiently transfected as above. C, levels of EZH2 mRNA were quantified by qRT-PCR (n = 4) and normalized to B2M in human fibroblasts transiently transfected with EZH2, WT1, anti-miR-26a, anti-miR-101, or their control plasmids. One-way analysis of variance with Dunnett's multiple comparison tests for each experimental cell line versus baseline cells transfected with EZH2 only: p < 0.0001 (****), p < 0.001 (***), not significant (ns).

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: WT1 cooperates with miRNAs to inhibit EZH2 expression. A, levels of EZH2 mRNA were quantified by qRT-PCR (n = 4) and normalized to β2-microglobulin (B2M) in human fibroblasts transiently transfected with EZH2, WT1, miR-26a, miR-101, or their control plasmids. B, levels of EZH2 protein were quantified by densitometric analysis of Western immunoblots normalized for β-actin (n = 4) in human fibroblasts transiently transfected as above. C, levels of EZH2 mRNA were quantified by qRT-PCR (n = 4) and normalized to B2M in human fibroblasts transiently transfected with EZH2, WT1, anti-miR-26a, anti-miR-101, or their control plasmids. One-way analysis of variance with Dunnett's multiple comparison tests for each experimental cell line versus baseline cells transfected with EZH2 only: p < 0.0001 (****), p < 0.001 (***), not significant (ns).

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Control, Western Blot, Comparison

    WT1 binds the 5′ UTR of EZH2. A, line drawing of the human EZH2 gene sequence showing four putative WT1 recognition motifs (GXGXGGXG) in the 5′ UTR (red boxes), and the 8-nucleotide seed sequences for miR-26a (UACUUGAA) and miR-101 (GUACUGUA) in the 3′ UTR (green boxes). B, electromobility shift assay showing a high molecular weight complex containing the 5′ UTR site (18-mer) bound to in vitro translated WT1(+KTS) protein (lanes 2 and 4). This complex disappears in the presence of excess cold probe (lane 3) or specific antibody against WT1 (lane 5). The high molecular weight complex formed remains intact with IgG antibody (lane 6). C, mRNA associated with exogenous WT1 protein was immunoprecipitated from fibroblasts transfected with EZH2 plasmid (RIP) and EZH2 RNA levels were quantified by qRT-PCR; WT1-associated EZH2 mRNA levels are expressed as percent of total mRNA input. D, endogenous EZH2 mRNA was immunoprecipitated with anti-WT1 antibody from a mouse mesonephric kidney cell line (M15 cells, black bars) and from mouse embryonic E17 kidneys (gray bars) and quantified by qRT-PCR. Unpaired t test, p < 0.0001 (****).

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: WT1 binds the 5′ UTR of EZH2. A, line drawing of the human EZH2 gene sequence showing four putative WT1 recognition motifs (GXGXGGXG) in the 5′ UTR (red boxes), and the 8-nucleotide seed sequences for miR-26a (UACUUGAA) and miR-101 (GUACUGUA) in the 3′ UTR (green boxes). B, electromobility shift assay showing a high molecular weight complex containing the 5′ UTR site (18-mer) bound to in vitro translated WT1(+KTS) protein (lanes 2 and 4). This complex disappears in the presence of excess cold probe (lane 3) or specific antibody against WT1 (lane 5). The high molecular weight complex formed remains intact with IgG antibody (lane 6). C, mRNA associated with exogenous WT1 protein was immunoprecipitated from fibroblasts transfected with EZH2 plasmid (RIP) and EZH2 RNA levels were quantified by qRT-PCR; WT1-associated EZH2 mRNA levels are expressed as percent of total mRNA input. D, endogenous EZH2 mRNA was immunoprecipitated with anti-WT1 antibody from a mouse mesonephric kidney cell line (M15 cells, black bars) and from mouse embryonic E17 kidneys (gray bars) and quantified by qRT-PCR. Unpaired t test, p < 0.0001 (****).

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques: Sequencing, Electro Mobility Shift Assay, High Molecular Weight, In Vitro, Immunoprecipitation, Transfection, Plasmid Preparation, Quantitative RT-PCR

    Interactions of WT1, miRNAs, and RISC components. A, representative Western blots (WB) probed with DICER, AGO2, or WT1 antibodies (left) of immunoprecipitates from amMSC versus amMSC/WT1(+KTS) using antibodies against DICER, AGO2, WT1, or control IgG (top). B, levels of EZH2 mRNA, miR-26a, and miR-101 were quantified by RIP-qPCR in amMSC expressing WT1(+KTS) (right bars) versus amMSC with empty vector (middle bars) or uninfected parental amMSC (left bars). Bar graphs show levels of miR26a (left upper), miR101 (right upper), and EZH2 (left lower) as percent of total RNA input. Two-way analysis of variance with Tukey's multiple comparison tests were used to compare WT1(+KTS) amMSC with empty vector control amMSC; p < 0.0001 (****), not significant (ns). C, representative Western blot probed with DICER1, AGO2, or WT1 antibodies (left) of RNase A-treated immunoprecipitates (top) from amMSC/WT1(+KTS) cells.

    Journal: The Journal of Biological Chemistry

    Article Title: Wilms Tumor Suppressor, WT1, Cooperates with MicroRNA-26a and MicroRNA-101 to Suppress Translation of the Polycomb Protein, EZH2, in Mesenchymal Stem Cells *

    doi: 10.1074/jbc.M115.678029

    Figure Lengend Snippet: Interactions of WT1, miRNAs, and RISC components. A, representative Western blots (WB) probed with DICER, AGO2, or WT1 antibodies (left) of immunoprecipitates from amMSC versus amMSC/WT1(+KTS) using antibodies against DICER, AGO2, WT1, or control IgG (top). B, levels of EZH2 mRNA, miR-26a, and miR-101 were quantified by RIP-qPCR in amMSC expressing WT1(+KTS) (right bars) versus amMSC with empty vector (middle bars) or uninfected parental amMSC (left bars). Bar graphs show levels of miR26a (left upper), miR101 (right upper), and EZH2 (left lower) as percent of total RNA input. Two-way analysis of variance with Tukey's multiple comparison tests were used to compare WT1(+KTS) amMSC with empty vector control amMSC; p < 0.0001 (****), not significant (ns). C, representative Western blot probed with DICER1, AGO2, or WT1 antibodies (left) of RNase A-treated immunoprecipitates (top) from amMSC/WT1(+KTS) cells.

    Article Snippet: Human fibroblasts (MCH070 cells) were transfected with pCMVSport6, pCMVSport6/hEZH2FL (insert size about 1.1 kb) (ATCC, MGC Human Clones, image ID: 3901250), WT1 cloned into a pcDNA3.1/Zeo(+) vector (kindly provided by Dr. P. Grundy, Calgary, AB, Canada), miScript miRNA mimics: Syn-hsa-miR-26a-5p, Syn-hsa-miR-101–3p, Syn-hsa-miR-93, and AllStars Negative Control siRNA, miScript miRNA inhibitor: anti-hsa-miR-26a-5p, anti-hsa-miR-101–3p, anti-hsa-miR-93, and miScript Inhibitor Negative Control (Qiagen) using Lipofectamine® 2000 transfection reagent according to the manufacturer's instructions (Invitrogen).

    Techniques: Western Blot, Control, Expressing, Plasmid Preparation, Comparison